Novel Cancer Vaccination Technology

Engineered Immunotherapy for Cancer

Our mission is to help patients fight cancer

It is the ultimate goal of CyTuVax to provide an innovative solution for the treatment of pancreatic cancer. CyTuVax has developed a revolutionary vaccination technology based on depot-attached cytokines that is capable of activating both the humoral and cellular arms of the immune system for a powerful anti-tumor response.
Rene Vleugels
Managing director

CyTuVax uses its adjuvant technology to develop both: prophylactic vaccines against viral & bacterial pathogens, and therapeutic vaccines against cancer. That is our mission, for which there is a high medical need. The patient is at the core in our vaccine development for which we apply our powerful adjuvant platform technology.

CyTuVax has developed a breakthrough, widely applicable and effective vaccination platform technology based on depot-attached cytokines as immune stimulator. The CyTuVax vaccines can generate powerful immune responses and are especially designed for cancer immunotherapy. The distinctive feature of our vaccine is a highly improved and prolonged antigen presentation by dendritic cells, which results in a stronger activation of immune cells.

The CyTuVax vaccination technology is a platform technology in several aspects. It can be applied with a wide range of cytokines aggregates as adjuvant and against a wide range of tumors. The vaccine has been successfully tested in preclinical studies with murine model tumors (fibro-sarcoma, melanoma, kidney, colon, mammary carcinoma and B-lymphoma). The effectiveness of the vaccination technology has also been verified in vaccines against viral targets (Hepatitis B) and bacterial targets (Antrax, Diphtheria and Tetanus).

CyTuVax is currently testing its vaccine technology in a clinical Phase 2 study of a vaccine for hepatitis B non-responders, persons that do not respond to standard hepatitis B vaccines. This clinical trial is being conducted with the Maastricht Universitair Medische Centrum (MUMC), Ziekenhuis Oost Limburg - Universiteit Hasselt, and Universiteit Antwerpen. More information about this clinical trial can be found at ClinicalTrials.gov.

Novel cancer vaccination platform technology

cytokine aggregates
Fig. 1: The CyTuVax vaccine: cytokine aggregates (green)
tumor cells (red) and tumor cell fragments (red).

CyTuVax has developed a powerful vaccination technology consisting of depot-attached cytokines as adjuvant and irradiated autologous tumor cells as vaccine target.

The distinctive feature of our vaccine is the highly improved antigen presentation by dendritic cells (DCs), which results in a strong activation of immune ‘killer’ cells. No other tumor vaccination technology is able to carry cytokine-micro-aggregates directly into the lymph node to improve and boost the activation of the patient’s immune cells. It is this direct stimulation of immune cells in the lymph node of the patient that induces the powerful immune response against the tumor.

The CyTuVax tumor vaccine consists depot-attached aggregated cytokine molecules as adjuvant and irradiated autologous tumor cells and/or specific tumor peptides as vaccine target.
A microscopic detail of the injection-ready CyTuVax vaccine is shown in Fig. 1. The adjuvant consists of several cytokines attached to a depot material in such a way that macro-aggregates are formed. After injection, these cytokine aggregates slowly break down into micro-aggregates and release “bursts” of cytokine aggregates. This release process is the decisive step for the efficiency of the CyTuVax’ vaccine.

cytokine aggregates
Fig. 2: Cytokine release pattern of the CyTuVax vaccine

Two factors are crucial in the built-up of a powerful and well-targeted anti-tumor immune response:
(1) the specifically engineered release process that mimics the natural release pattern of cytokines by immune cells. When communicating with other immune cells, cytokine molecules are released from vesicles into the cell’s environment. This spray-like release of cytokines attracts an influx of cells of the innate immune system, among others dendritic cells, to the injection site.
Fig.2 shows how these bursts of small cytokine aggregates (small green dots) attract DCs, the main players of immune response induction. With their receptors, the DCs are able to bind these cytokines. The DCs migrate to the nearest lymph node draining the inoculation site to deposit these cytokines.

(2) the release process of cytokines takes place over a long period of time. This means that the vaccines are active for a long period and capable of constantly attracting DCs to the inoculation site.

Inside the lymph nodes, DCs present peptides of the cancer antigen fragments from the autologous tumor cells and deposit cytokine aggregates in the lymph node’s extra cellular matrix. This process forcefully stimulates the T cells and NK cells into action. A powerful immune response is followed as the cytokine-aggregates decompose inside the lymph nodes (see Fig. 3). The specifically activated T cells and NK cells then leave the lymph nodes to search and destroy tumor cells wherever these can be found in the patient’s body.

dendritic_cells
Fig. 3a) CD86+ dendritic cells (red/yellow stain) Fig. 3b) This shows the IL-2 micro-aggregates (green) carried and deposited into the lymph node by dendritic cells.

Contrary to other immunotherapy concepts using in vitro engineered autologous dendritic cells, the attraction and maturation of DCs is achieved in situ, at the inoculation site, under the influence of the released cytokines, and not in vitro in tissue culture. This approach sets the CyTuVax’ technology apart from its competitors and overcomes a major weakness of in vitro stimulated cells: their inability to migrate to the lymph nodes. Our vaccines stimulate the ‘natural’ activation and maturation processes whereby matured DC’s have kept their full migratory powers and reach the lymph node to initiate a powerful anti-tumor response by killer cells.

The CyTuVax vaccination technology is a platform technology in several aspects. It can be applied with a wide range of cytokines and other immune-stimulating agents and against a wide range of tumors.

The vaccine has been successfully tested in preclinical studies with murine model tumors (fibro-sarcoma, melanoma, kidney, colon, mammary carcinoma and B-lymphoma).

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showCyTuVax is currently testing its vaccine technology in a clinical Phase 2 study of a vaccine for hepatitis B non-responders

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About us

CyTuVax B.V.
Oxfordlaan 55
6229 EV Maastricht
The Netherlands

+31-433881424
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